Title Utjecaj prenatalne izloženosti niskim koncentracijama metil-žive na razvoj mozga u novorođenčadi : doktorski rad Title (english) Prenatal low-level methylmercury exposure and development of brain in newborn Author Iva Bilić Čače Mentor Igor Prpić (mentor)Committee member Antun Sasso (predsjednik povjerenstva)Committee member Jadranka Mustajbegović (član povjerenstva)Committee member Vladimir Mićović (član povjerenstva)Committee member Igor Prpić (član povjerenstva)Granter University of Rijeka Defense date and country 2011-01-01, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Universal decimal classificationUDC ) 615 - Pharmacology. Therapeutics. Toxicology Abstract Ciljevi istraživanja: Izrada percentilnih krivulja koncentracija metil-žive u organizmu majke i novorođenčeta s obzirom na učestalost konzumacije ribe tijekom trudnoće. Usporedba konzumacije ribe i plodova mora s vrijednostima tjelesne koncentracije metil-žive. Ispitati dovodi li konzumacija ribe i morskih plodova uobičajenom prehranom trudnica obalne regije Jadranskog mora do neurorazvojnih i/ili morfoloških promjena mozga novorođenčeta. Utvrditi valjanost dvaju bioloških uzoraka- kose majke i krvi pupkovine.
Ispitanici i metode: Koncentracija ukupne žive izmjerena je u kosi majke i u miješanoj krvi pupkovine. U ispitanica kojima je koncentracija ukupne žive uzorcima kose iznosila ≥ 1,0 µg/g izmjerena je i koncentracija metil-žive u oba biološka uzorka. Na temelju koncentracije žive u uzorcima kose definirana je izložena (≥ 1,0 µg/g) i neizložena (< 1,0 µg/g) skupina. Izloženost fetusa živi putem majčine konzumacije hrane morskog porijekla ispitana je upitnikom prehrane. Svoj je dostupnoj novorođenčadi učinjen ultrazvučni pregled mozga i standardizirani neurološki test Amiel- Tison.
Rezultati: Medijan i IK raspon koncentracija metil-žive u kosi majke iznosi 608 ng/g (288,5- 957,5 ng/g). Medijan i IK raspon koncentracija metil-žive u krvi pupkovine iznosi 2,9 ng/g (1,4-5,6 ng/g). Postoji izvrsna povezanost koncentracije metil-žive u dva ispitivana biološka uzorka (rho=0,88). Postoji slaba do umjerena pozitivna korelacija između konzumacije ribe i tjelesnih koncentracija metil-žive (rho=0,25-0,45). Od vrste ribe relativno najviše se konzumira tuna. Dokazano je postojanje slabe do umjerena pozitivne korelacija između konzumacije pojedine vrste ribe i tjelesnih koncentracija metil-žive (rho=0,22-0,46). Postoji statistički značajna razlika u duljini cerebelluma (P=0,013) između izložene i neizložene skupine, a razlike u širini cerebelluma (P= 0,057) i širini gyrus frontalis superiora (P= 0,052) među skupinama jednake su granicama značajnosti. Rezultati Amiel-Tison testa u svih ispitanika bili su potpuno jednaki i odgovaraju u cijelosti urednom nalazu. Zaključci: Izmjerena koncentracija metil-žive žive u oba biološka uzorka u potpunosti se podudara sa izmjerenom koncentracijom ukupne žive. Mjerenjem u bilo kojem od dva biološka uzorka možemo podjednako zaključivati o utjecaju žive na ishod novorođenčeta. S porastom konzumacije ribe raste i tjelesna koncentracija metil-žive. Postoji povezanost između tjelesne koncentracije metil-žive te konzumacije ribe i dimenzija određenih struktura mozga. Postoje razlike u duljini i širini cerebelluma te širini gyrus frontalis superiora s obzirom na graničnu vrijednost koncentracije metil-žive od 1,0 µg/g.
Abstract (english) Objectives: To create the percentile curves of methylmercury body concentrations in relation to frequency of fish consumption during pregnancy. To compare fish and sea-food consumption with body concentrations of methylmercury. To determine whether the usual diet of pregnant women living in the coastal area of the Adriatic Sea, particularly fish and sea-food consumption, influences neurodevelopment and/or leads to morphologic alterations of the newborns brain. To validate used biologic samples, maternal hair and cord blood.
Patients and Methods: Total mercury concentration has been measured in hair and mixed cord blood. Methylmercury concentration has been measured in hair and cord blood of the patients whose total mercury concentration was ≥ 1,0 µg/g. According to the hair methyl-mercury concentration values, examinees are divided into exposed (≥ 1,0 µg/g) and non-exposed (< 1,0 µg/g) group. Maternal sea-food consumption has been assessed by written food questionnaire. Brain ultrasound and neurological Amiel- Tison test have been conducted to all newborns.
Results: Maternal hair methylmercury concentration median and inter-quartile range was 608 ng/g (288,5- 957,5 ng/g). Cord blood methylmercury concentration median and inter-quartile range was 2,9 ng/g (1,4-5,6 ng/g). There is an excellent correlation between methylmercury concentrations measured in both biologic samples (rho=0,88). There is weak to moderate correlation between fish consumption and body methylmercury concentrations (rho=0,25-0,45). Tuna is most frequently consumed type of fish. There is weak to moderate correlation between consumption of particular type of fish and body methylmercury concentrations (rho=0,22-0,46). There is statistically significant difference between length of cerebellum (P=0,013) between exposed and non-exposed group. Differences in the width of cerebellum (P= 0,057) and the width of the gyrus frontalis superior (P= 0,052) are at the borderline of significance. Amiel-Tison test results showed identical, normal neurological status in all the examinees.
Conclusions: Methylmercury concentration is equal to total mercury concentration in both biological samples. Both biological samples are equally valid vehicles for measuring prenatal methylmercury exposure. With the increase in fish consumption, the body methylmercury concentration increases. There is correlation between body methylmercury concentration, fish consumption, and particular brain structures' dimensions. There are differences between width and length of cerebellum, and width of gyrus frontalis superior in relation to methylmercury cut-off limit of 1,0 µg/g.
Keywords
metil-živa
mozak
novorođenče
prenatalna izloženost
Keywords (english)
methylmercury
brain
newborn
prenatal exposure
Language croatian URN:NBN urn:nbn:hr:188:074536 Project Number: 062-0000000-3395 Study programme Title: Biomedicine Postgraduate (doctoral) study programme Catalog URL https://libraries.uniri.hr/cgi-bin/unilib.cgi?form=D1121018039 Type of resource Text Extent 119 str. File origin Born digital Access conditions Closed access Terms of use Created on 2017-01-19 17:52:37
