prikaz prve stranice dokumenta T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima
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doctoral thesis
T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima
komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora
1985. urn:nbn:hr:188:342423
Jonjić, Stipan
University of Rijeka
Faculty of Medicine

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Jonjić, S. (1985). T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora (Doctoral thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:188:342423

Jonjić, Stipan. "T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora." Doctoral thesis, University of Rijeka, Faculty of Medicine, 1985. https://urn.nsk.hr/urn:nbn:hr:188:342423

Jonjić, Stipan. "T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora." Doctoral thesis, University of Rijeka, Faculty of Medicine, 1985. https://urn.nsk.hr/urn:nbn:hr:188:342423

Jonjić, S. (1985). 'T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora', Doctoral thesis, University of Rijeka, Faculty of Medicine, accessed 06 December 2024, https://urn.nsk.hr/urn:nbn:hr:188:342423

Jonjić S. T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora [Doctoral thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 1985 [cited 2024 December 06] Available at: https://urn.nsk.hr/urn:nbn:hr:188:342423

S. Jonjić, "T limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora", Doctoral thesis, University of Rijeka, Faculty of Medicine, Rijeka, 1985. Available at: https://urn.nsk.hr/urn:nbn:hr:188:342423

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TitleT limfocitne subpopulacije u svinje karakterzirane monoklonskim protutijelima : komparativna analiza s homolognim subpopulacijama u čovjeka, miša i štakora
AuthorStipan Jonjić
MBZ: 95983
MentorDanijel Rukavina (mentor)
MBZ: 41705
MentorBiserka Radošević-Stašić (komentor)
MBZ: 39764
Committee memberDanijel Rukavina (predsjednik povjerenstva)
MBZ: 41705
Committee memberBiserka Radošević-Stašić (član povjerenstva)
MBZ: 39764
Committee memberBranko Vitale (član povjerenstva)
MBZ: 52531
GranterUniversity of Rijeka
Faculty of Medicine
Rijeka
Defense date and country1985-12-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Immunology
Universal decimal classification
(UDC)		612 - Physiology
Abstract
U radu je opisana produkcija i karakterizacija monoklonskih antitijela (mAt), za antigene na limfocitima svinje. Antitijela su produkti hibridoma nastalih fuzijom SP2/0 mijeloma i splenocita BALB/c, miša imuniziranog s timocitima svinje. Dva mAt, 122/28 i 295/33 prepoznaju antigen nazvan S-L 2, koji je pri- sutan na populaciji i supresorskih limfocita i homologan je s T-8 antigenom u ljudi, Lyt-2 antigenom u miša i MRC 0X-8 u štakora. S-L 2 antigen je od dva polieptidna lanca, molekularne težine od 33-35 kd u reduciranim uvjetima i 65-70 kd u nereduciranim uvjetima. rnAt 122/28 i 295/33 vežu se na 30-40% limfo- cira iz periferne krvi, a nereaktivna su s B limfocitima, monocitima i granulocitima kao i stanicama koštane srži. Eliminacijom S-L 2+ stanica mAt 122/28 i 295/33 i komplementa se smanjuje proliferativni odgovor limfocita na T mitogene (Con- canavali-A i Pokeweed mitogen), a odgovor na B mitogene (Lipopoli- saharid i Staphylococcus aureus) nije bio narušen. Identičan tretman limfocita nije imao utjecaja na proliferativni odgovor na aloantigene u reakciji pomiješanih limfocita (MLR). S-L 2 antigen je prisutan na prekursorima i efektorskim CTL. Inkubacijom limfocita s mAt 122/28 i 295/33 tijekom MLR, potpuno se sazrijevanje cito-toksičnih T limfocita, a inkubacijom zrelih citotoksičnih T limfocita, akoči se njihova citoksična aktivnost.
Abstract (english)
In this work the production and characterization of monoclonal antibodies (mAb) that are specific for swine lymphocytes are described. The antibodies resulted from a fusion between SP2/0 myeloma cells and spleen lymphocytes from mice immunized to pig thymocytes. Two mAb, 295/33 and 122/28, recognized an antigen termed S-L 2 with an apperent m.w. of 33 to 35 kilodaltons under reducing conditions and 70 kilo- daltons under nonreducing conditions. This antigen is present on cyto- lytic and suppressor T cells and seems to be a swine homologe of the T-8 antigen in human, Lyt-2 in mouse and MRC 0X-8 in rat. mAb 122/28 and 295/33 were reactive in indirect immunofluroescence with 30-40% of peripheral blood lymphocytes but they were nonreactive with bone marrow cells, Ig+B cells, granulocytes and monocytes. In functional studies, the elimination of S-L 2+ cells with mAb and complement, partially reduced the proliferative response to T-cell mitogens (Concanavalin-A and Pokeweed mitogen) but not to 8-cell mitogens (Staphilococcus aureus and Lipopolysaccharide). Identical treatment did not influence the proliferative response to alloantigens in MLR. Both, cytotoxic T effector and precursor cells carried the antigen S-L 2. mAb 122/28 and 295/33 without complement were able to block the generation of CTL in MLR when added to the cultures. They were also capable of blocking the lytic activity by CTL when cells were incubated with antibodies before testing their lytic activity in CML.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:188:342423
Promotion1985-12
Study programmeTitle: Biomedicine Postgraduate (doctoral) study programme
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Catalog URLhttps://libraries.uniri.hr/cgi-bin/unilib.cgi?form=D1100302089
Type of resourceText
Extent161 str
File originReformatted digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2017-01-19 18:59:45